Berberine indicated much higher activity against promastigote forms than crude. Dec 31, 2007 the stilbene, piceatannol, was screened for its antileishmanial activity against promastigotes as an extracellular form of leishmania donovani, leishmania infantum and leishmania major, and amastigotes of leishmania donovani as an intracellular form. The cannabiniods extracts showed the antioxidant activity by changing to yellow colour. Cytotoxicity of these plant extracts was also assessed. Antimicrobial activity of crude extracts of three medicinal. Sixteen endophytes were isolated by using potato dextrose agar medium and submitted to cultivation in rice medium. In the present study, the in vitro antidermatophyte, antileishmanial, and cytotoxicity activities of various extracts of b. Antileishmanial activity of moringa oleifera leaf extracts. Evaluation of in vitro antileishmanial activity of extracts and determination phenolic contents. Screening of in vitro antileishmanial activity of extracts of selected medicinal plants. Medicinal plants with promising antileishmanial activity in iran. Antifungal, antileishmanial, and cytotoxicity activities.
Annals of clinical cytology and pathology antileishmanial. In vitro antileishmanial activity of mexican medicinal plants ncbi. In vitro antileishmanial activity of methanolic and. Different solvent extracts from ten plants were colorimetrically tested against the promastigotes.
Immunomodulatory, antileishmanial and phytotoxicity of. Antileishmanial activity of medicinal plants used in. Screening of in vitro antileishmanial activity of extracts of. The cytotoxic and antileishmanial activity of extracts and fractions of leaves and fruits of azadirachta indica a juss. Of these extracts, the fruit of brucea javanica was the most active in inhibiting parasite growth at a concentration of 10 gml. Antileishmanial potential of medicinal plant extracts from the north. Six different extracts of melodinus eugeniifolus a very rare medical plant belongs to apocynaceae leaves and barks were screened for the in vitro antileishmanial and antibacterial activities, among the different extracts tested, the ethanol and.
All plants extracts did not show deleterious activity to the host cell evidenced by ldh assay at 100, 10, and 1. The aim of this study is to evaluate the antileishmanial activity of selected medicinal plants from the northwest of morocco. In vitro antileishmanial activity of leaf and stem. Pdf antileishmanial activity of selected turkish medicinal. Antileishmanial activity of aloe secundiflora plant. Due to our continuous search for new alternatives for the treatment of leishmaniasis, this study aimed to investigated the leishmanicidal activity of 17 plant methanolic extracts against four leishmaniaactivity was determined by the colorimetric. Phytochemical analysis and antileishmanial activity of. Antileishmanial activity was performed to expose potential in inhibiting and retarding multiplication of leishmanial parasite. Mar 10, 2014 in the present study, the in vitro antidermatophyte, antileishmanial, and cytotoxicity activities of various extracts of b. In vitro antileishmanial activity of mexican medicinal plants. The fungal cultures were extracted with ethanol and used as crude extracts. Plant extracts were prepared by maceration using methanol, ethanol. Antifungal, antileishmanial, and cytotoxicity activities of.
Screening of indonesian medicinal plant extracts for. Firstly, as a preliminary assay of leishmanicidal activity, all the extracts were tested against leishmania amazonensis promastigotes. Antileishmanial activity of selected turkish medicinal plants. In this study, we tested the leishmanicidal activity of thirty extracts obtained from ten plants used in mexican traditional medicine as antiparasitics. Research article antifungal, antileishmanial, and cytotoxicity activities of various extracts of berberis vulgaris berberidaceae and its active principle berberine hosseinmahmoudvand, 1,2 seyyedaminayatollahimousavi, 3 asgharsepahvand, 4 faribasharififar, 5 behrouzezatpour, 2 fatemehgorohi, 6 ebrahimsaedidezaki, 3 andsarehjahanbakhsh 1,2. Compared with previous studies which have been reported on the antileishmanial activity against l.
Boiled extracts derived from 28 indonesian medicinal plants were screened for their antibabesial activity against babesia gibsoni in vitro. The chloroform extract of the roots of the plant have shown. Evidencebased complementary and alternative medicine hindawi. The results of antimicrobial activity showed that water and ethanol crude extracts were active on all the strains of pathogens tested at different concentrations, with ethanol extracts exerting more activity. Antileishmanial activity of medicinal plants used in endemic areas. Research article antifungal, antileishmanial, and cytotoxicity activities of various extracts of berberis vulgaris berberidaceae and. The aim of this study was to evaluate in vitro the antileishmanial activities of organic extracts methanol, nhexane and dichloromethane extract from salvia clandestina lamiaceae used in moroccan medicinal plant. The results of the inhibition percentages for all plant extracts used in general antileishmanial screening test at 50. Pdf antileishmanial activity of medicinal plants from africa. Pdf the aim of this study is to evaluate the antileishmanial activity of selected medicinal plants from the northwest of morocco. Cannabinoids, plant extracts, crude extracts, anibacterial activity, antifungal activity, antioxidant activity. Evaluation of antileishmanial activity of albaha medicinal. Its appearance was observed varying with polarity of solvent used for fractionation. Introduction marijuana cannabis sativa has long been known to.
Pentavalent antimony compounds are expensive, toxic and drug resistance is prevalent, whereas the plant extract derivatives are safe. Different fractions of methanol extract of the plants used in this study indicated that jdee of j. Table gives the names of the plants, family, parts investigated, voucher specimen numbers, local names, yields in %, and their traditional uses. The purpose of this work was to evaluate the antileishmanial activity of endophytic fungi isolated from leaves of vernonia polyanthes plant and their prospective use in the discovery of bioactive compounds. Extraction of cannabinoids from cannabis sativa l plant and. Bioassayguided fractionation of the fruit extract of br. All plants extracts did not show deleterious activity to the host cell evidenced by ldh assay at, and gml a. In conclusion, this preliminary study led to the identification of seven plant extracts, namely, a. A series of ethnopharmacological surveys conducted in brazil recorded the traditional use of plants against different pathologies and interestingly, some of them presented antileishmanial activity in vitro and in vivo, possibly due to their immunostimulatory, healing and microbicidal properties. Hplc analysis of supercritical carbon dioxide and compressed. Antileishmanial activity of the hydroalcoholic extract of. Methodology procurement and authentication of plant material plant material was collected from hills of bihar and.
Annals of clinical cytology and pathology antileishmanial potential of crude plant extracts derived from medicinal plants in palestine. Apr 20, 2012 to determine in vitro antileishmanial activity, 3. To the best of our knowledge, this is the first experimental report of the antileishmanial activity ofplumeria pudicaleaf. In the present study, the antileishmanial activity of agave americana a xerophitic plant of genus agave of indian brought up was evaluated. Moderate antipromastigote activity with inhibition of parasite growth in the range of 3040 % was witnessed in the extracts of asparagus racemosus, boerhaavia diffusa, citrullus colocynthis, spilanthes acmella and thuja occidentalis. Pdf antileishmanial potential of medicinal plant extracts from the. Phytochemical evaluation and antimicrobial activity of crude. Several organic extractsessential oils and their constituents have been tested in some african countries for their antileishmanial activities.
Antileishmanial activity was performed against leishmania tropica kwh23 promastigote. Research article evaluation of antileishmanial activity of. Antileishmanial, toxicity, and phytochemical evaluation of. Pdf antileishmanial potential of tropical rainforest. Pdf antileishmanial activity of aloe secundiflora plant. Melodinus eugeniifolus, a very rare medical plant which belongs to the family of apocynaceae, has just been discovered in the malaysia rain forest. Research article antileishmanial activity of medicinal. In the investigation of antileishmanial effects of b. Antileishmanial activity of tested plant extract against, leishmania donovani strain mhomin1983ag83 promastigotes, was evaluated by a quantitative colourimetric assay using mtt 34,5dimethylthiazol2yl2, 5diphenyl tetrazolium bromide 7.
Results of antileishmanial activity, cytotoxic e ects on peritoneal macrophage from balbc mice, and selectivity of methanolic extracts studied are. Evidencebased complementary and alternative medicine. Aim of the study to evaluate the antileishmanial activity and cytotoxicity of aqueous and organic extracts of ten plants used in mexican traditional medicine as antiparasitics. In vitro parasite inhibition by the plant extracts ranged between 16.
A b s t r a c t chemical profiles of extracts of four species from laurencia complex ceramiales, rhodophyta from different populations collected along southeast brazilian coast were assessed by high performance liquid chromatography coupled with a. In vitro antiplasmodial, antileishmanial and antitrypanosomal. In this study antileishmanial activity of the methanolic and water leaf extracts from aloe secundiflora plant were analysed by determining the minimum inhibition concentration mic, nitric oxide. The aim of this study was to investigate the antileishmanial activity of the 16 methanol extracts from selected plant species from albaha region, against leishmania amazonensis. Therefore, from the present study it can be inferred that p. The methanol extract was the most active followed by chloroform and petroleum ether extracts. In this study, all tested extracts exhibited antileishmanial activity after. Evaluation of the in vitro antiplasmodial, antileishmanial, and antitrypanosomal activity of medicinal plants used in saudi and yemeni traditional medicine ramzia. Evaluation of the in vitro antiplasmodial, antileishmanial.
Antileishmanial activities of the chloroform and methanol extracts were evaluated by 96well micro titer plate method. In the present study, aqueous extract of all the plants gave some level of activity against the promastigotes. Plant extracts from various species of the genus piper have demonstrated potent antileishmanial efficacy and leishmanicidal molecules apart from piperine have also been identified 63. Antileishmanial activity of peganum harmala extract on the. In vitro antileishmanial activity against leishmania donovani promastigotes.
The cytotoxic activity against normal peritoneal macrophages from normal balbc mice was also determined. Screening of in vitro antileishmanial activity of extracts. In vitro antileishmanial activity of pleumeria pudica leaf. Antileishmanial potential of tropical rainforest plant extracts. All the extracts displayed remarkable antibacterial activity against sta. In addition to in vitro antileishmanial activity against leishmania tropica promastigotes, cytotoxic activity of the plant extract was also measured using a wst1 cell proliferation assay 11, 12.
The methanolic extracts of these plants were screened for their antileishmanial activity against leishmania major using 34. Pdf chemical diversity and antileishmanial activity of. Prediluted plant extracts were subsequently added and the plates were further incubated for 5 days at 37c and 5% co 2. Antileishmanial promastigotes leishmania donovan plumeria pudica. In vitro antileishmanial activity of extracts from endemic. In addition, the extracts were screened for cytotoxic activity against balbc mouse macrophages in order to assess a selectivity index.
The cytotoxic and antileishmanial activity of extracts and. Antileishmanial activity of piceatannol isolated from. Antileishmanial potential of medicinal plant extracts from. The chloroform extract of the roots of the plant have shown significant antileishmanial activity while the methanol extracts of the shoots and fruits have shown low. This study investigates the leishmanicidal activity of five species of plants used in folk medicine in endemic areas of the state of alagoas, brazil. Research on azadirachta indica fractions and extracts has shown that they have excellent antileishmanial activity based on bioactivityguided fractionation of ethanolic extracts of leaves and seeds and in vitro activity against promastigotes. This prompted us to evaluate the antileishmanial efficacy of nigrum p.
A total of 115 different plant extracts from our collection, representing 96 plant species, have been screened for in vitro antileishmanial activity against l. The plants were extracted with methanol and screened in vitro against erythrocytic schizonts of plasmodium falciparum, intracellular amastigotes of leishmania infantum and trypanosoma cruzi, and free trypomastigotes of t. Hplc analysis of supercritical carbon dioxide and compressed propane extracts from piper amalago l. Study of the in vitro antiplasmodial, antileishmanial and. Research article open access in vitro antiplasmodial. Antileishmanial potential of piper nigrum seed extracts. Evaluation of leishmanicidal effect of euphorbia petiolata extract by in vivo. Aug 29, 2015 the purpose of this work was to evaluate the antileishmanial activity of endophytic fungi isolated from leaves of vernonia polyanthes plant and their prospective use in the discovery of bioactive compounds. To evaluate the antileishmanial activity and cytotoxicity of aqueous and organic extracts of ten plants used in mexican traditional medicine as antiparasitics. To evaluate the antileishmanial activity of the plants. In a previous work, we reported the antileishmanial activity of extracts of 20 plants from the brazilian flora braga et al. In the present study, the effect of methanolic and aqueous extracts of eucalyptus camaldulensis on the promastigotes of leishmania major was evaluated. For the organic extracts, plant material was macerated in dichloromethane.
The current study is the first scientific account on the significant antileishmanial activity ic 50. It is noteworthy to mention that to the best of our knowledge, this study represents the first report on antileishmanial activity for some of the tested plants. Alajmi, 1 paulcos, 3 andlouismaes 3 department of pharmacognosy, college of pharmacy, king saud university, p. In this study antileishmanial activity of the methanolic and water leaf extracts from aloe secundiflora plant were analysed by determining the minimum inhibition concentration mic, nitric oxide no production stimulation, infection rates ir and multiplication index mi. Clerodendrum myricoides and salvadora persica have been reported in management of leishmaniasis which is one of the neglected tropical diseases. Antileishmanial activity of tussilago farfara carum copticum. Potent antileishmanial activity was observed for j. Antileishmanial activity in crude extract of seaweeds 165 antileishmanial bioassay.
Sixteen methanolic extracts obtained from thirteen plant species, selected either from ethnobotanical or chemotaxonomical data, were screened for their antileishmanial activity against leishmania amazonensis. Antileishmanial activity of some brazilian plants, with. Torressantos 3 and diogenes aparicio garcia cortez 1. Antileishmanial assay and antimicrobial activity on crude. Antileishmanial activity of aloe secundiflora plant extracts. The antiplasmodial, antileishmanial, and antitrypanosomal activity of twentyfive medicinal plants distributed in saudi arabia and yemen was evaluated. The study evaluated the antileishmanial activity of four medicinal plants collected from the northwest of morocco. Research article evaluation of the in vitro antiplasmodial. The antileishmanial activity exhibited by plant extracts has been attributed to compounds belonging to different groups of secondary metabolites chanbacab and penarodrigues, 2001, rocha et al. Antileishmanial activity of extracts from libidibia ferrea.
In general, nonpolar and moderately polar extracts were found to be more active than polar extracts, except. In vitro antileishmanial activity of methanolic extracts for some. The study aimed at undertaking phytochemical analysis as well as investigating the in vitro antileishmanial activity of clerodendrum myricoides and salvadora persica extracts on promastigote and amastigote forms of leishmania major. Plant extracts were prepared by maceration using methanol, ethanol, and nhexane. Due to our continuous search for new alternatives for the treatment of leishmaniasis, this study. Medicinal plant extracts are rich in phenolic contents such as polyphenol and flavonoids.
Antileishmanial potential of tropical rainforest plant. Antileishmanial activity of compounds produced by endophytic. In addition, the extracts were screened for cytotoxic activity against balbc mouse macrophages in. The plant kingdom is undoubtedly valuable as a source of new medicinal agents. However, further phytochemical andin vivostudies and. Research article antileishmanial activity of medicinal plants used in.
Crude extracts that showed a selectivity index cc50 for macrophage. A total of 115 different plant extracts from our collection, representing 96 plant species, have been evaluated for in vitro antileishmanial activity against l. As part of our ongoing research on plant derived metabolites for the treatment of diseases caused by protozoan parasites 12,20, evaluation of the in vitro antileishmanial activity of the hydroalcoholic extract of miconia langsdorffii cogn. Antileishmanial activity of selected south african plant. Extracts and secondary metabolites exhibiting antileishmanial activity.